Journal: Oncoimmunology

Article Title: T cells drive negative feedback mechanisms in cancer associated fibroblasts, promoting expression of co-inhibitory ligands, CD73 and IL-27 in non-small cell lung cancer

doi: 10.1080/2162402X.2021.1940675

Figure Lengend Snippet:

Article Snippet: IL-27 Goat anti-Human, Polyclonal, R&D SystemsTM , RnD systems , RnD systems Cat# Af2526 Goat anti-human polyclonal antibody.

Techniques: Purification, Control, Activation Assay, Blocking Assay, Enzyme-linked Immunosorbent Assay, Recombinant, Cell Stimulation, Red Blood Cell Lysis

Journal: Scientific Reports

Article Title: Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation

doi: 10.1038/s41598-018-28614-4

Figure Lengend Snippet: The expression of sIL-27Rα level in GVHD patients. ( A ) Serum sIL-27Rα levels were significantly increased on the day of neutrophil engraftment compared with pre-conditioning ( P < 0.0001). ( B ) The serum sIL-27Rα levels in patients with grade II–IV aGVHD were significantly lower than those of 0-I aGVHD patients on the day of neutrophil engraftment ( P < 0.001). ( C ) On the day of neutrophil engraftment, serum sIL-27Rα levels in patients with skin aGVHD ( P < 0.01) and liver aGVHD ( P < 0.05) were significantly lower than those of 0-I aGVHD patients. ( D ) There were no similar significant difference regarding cGVHD. Data shown are mean ± SD.

Article Snippet: After washing, biotinylated goat anti-human IL-27Rα polyclonal antibody (100 μL/well, Catalogue #BAF1479, R&D Systems) was added as detection antibody for 2 h at room temperature.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation

doi: 10.1038/s41598-018-28614-4

Figure Lengend Snippet: The diagnostic value of sIL-27Rα in aGVHD and the association of sIL-27Rα with aGVHD severity, relapse and survival. ( A ) The area under the ROC curve (AUC) was 0.735 (95% CI 0.618–0.853, P = 0.001) on the day of neutrophil engraftment; when using 59.4 ng/ml as cut-off value from the ROC curve, the sensitivity and specificity were 56% and 81%, respectively. ( B ) The cumulative incidence of grade II–IV aGVHD was significantly lower in patients with high sIL-27Rα levels by Gray’s test ( P = 0.004). ( C ) Patients with high sIL-27Rα levels showed favourable overall survival compared with patients with low sIL-27Rα levels with Kaplan-Meier survival analysis by log rank test ( P < 0.001). ( D , E ) Patients with high sIL-27Rα levels had lower relapse rate (CIR) and non-relapse mortality (NRM) than did patients with low sIL-27Rα levels by Gray’s test (P = 0.008, respectively).

Article Snippet: After washing, biotinylated goat anti-human IL-27Rα polyclonal antibody (100 μL/well, Catalogue #BAF1479, R&D Systems) was added as detection antibody for 2 h at room temperature.

Techniques: Diagnostic Assay

Journal: Scientific Reports

Article Title: Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation

doi: 10.1038/s41598-018-28614-4

Figure Lengend Snippet: The association of sIL-27Rα levels at neutrophil engraftment with clinical factors.

Article Snippet: After washing, biotinylated goat anti-human IL-27Rα polyclonal antibody (100 μL/well, Catalogue #BAF1479, R&D Systems) was added as detection antibody for 2 h at room temperature.

Techniques: Biomarker Discovery

Journal: Scientific Reports

Article Title: Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation

doi: 10.1038/s41598-018-28614-4

Figure Lengend Snippet: Univariate and multivariate analyses of factors affecting the incidence of grade II–IV acute graft- versus-host disease after allogeneic hematopoietic stem cell transplantation at neutrophil engraftment.

Article Snippet: After washing, biotinylated goat anti-human IL-27Rα polyclonal antibody (100 μL/well, Catalogue #BAF1479, R&D Systems) was added as detection antibody for 2 h at room temperature.

Techniques: Transplantation Assay

Journal: Scientific Reports

Article Title: Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation

doi: 10.1038/s41598-018-28614-4

Figure Lengend Snippet: sIL-27Rα as an independent prognostic aGVHD biomarker validated in an independent cohort of 80 patients. ( A ) The area under the ROC curve (AUC) was 0.790 (95% CI 0.688–0.892, P < 0.001) on the day of neutrophil engraftment, using 59.4 ng/ml as the cut-off value. ( B ) The cumulative incidence of grade II–IV aGVHD was significantly lower in patients with high sIL-27Rα levels by Gray’s test ( P < 0.001). ( C ) Patients with high sIL-27Rα levels showed favourable overall survival compared with patients with low sIL-27Rα levels on Kaplan-Meier survival analysis by log rank test ( P = 0.012). ( D , E ) Patients with high sIL-27Rα levels had similar relapse rates (CIR) and lower non-relapse mortality (NRM) than did patients with low sIL-27Rα levels by Gray’s test ( P = 0.0931, P = 0.005, respectively).

Article Snippet: After washing, biotinylated goat anti-human IL-27Rα polyclonal antibody (100 μL/well, Catalogue #BAF1479, R&D Systems) was added as detection antibody for 2 h at room temperature.

Techniques: Biomarker Discovery

Journal: Scientific Reports

Article Title: Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation

doi: 10.1038/s41598-018-28614-4

Figure Lengend Snippet: Association of sIL-27Rα with serum cytokine levels in aGVHD. The associations between sIL-27Rα levels with serum IL-27, IL-10, HGF, TNFR1, elafin, REG3α and ST2 levels were analysed by using Spearman’s rank correlation coefficient.

Article Snippet: After washing, biotinylated goat anti-human IL-27Rα polyclonal antibody (100 μL/well, Catalogue #BAF1479, R&D Systems) was added as detection antibody for 2 h at room temperature.

Techniques:

Journal: Mediators of Inflammation

Article Title: IL-27 Activates Human Trophoblasts to Express IP-10 and IL-6: Implications in the Immunopathophysiology of Preeclampsia

doi: 10.1155/2014/926875

Figure Lengend Snippet: Analysis of IL-27 and IL-27 receptor expression in placental tissues. (a) Representative Western blot analysis of IL-27 receptor subunits WSX-1 in placental tissues. The first three are representative of samples of normal pregnancy (N) group and the last is from the PE group. (b)–(i) Immunohistochemical staining of IL-27 and WSX-1 in the placental tissues. (b) and (d) show the immunostaining of IL-27 in normal control (N) group; (c) and (e) show the immunostaining of IL-27 in preeclampsia (PE) group. (f) and (h) show the immunostaining of the WSX-1 in normal control (N) group; (g) and (i) show the immunostaining of WSX-1 in preeclampsia (PE) group. β -Actin was used as protein control to ensure an equal amount of loaded protein. Original magnification: 100x for c, f, g, and h; 400x for d, e, h, and i. All experiments were performed in three independent replicates.

Article Snippet: Sections were incubated with anti-IL-27 mAb (Abcam115671, HK) and anti-WSX-1 mAb (RD AF1479), diluted in 5% (wt/vol) nonfat milk for 16 h at 4°C.

Techniques: Expressing, Western Blot, Immunohistochemical staining, Staining, Immunostaining

Journal: Mediators of Inflammation

Article Title: IL-27 Activates Human Trophoblasts to Express IP-10 and IL-6: Implications in the Immunopathophysiology of Preeclampsia

doi: 10.1155/2014/926875

Figure Lengend Snippet: IL-27 and IL-27 receptor was expressed in HTR-8/SVneo cells and it upregulated CXCL10 and IL-6. (a) RNA and protein expression for WSX-1. (b) RNA and protein expression of gp130. From the left to the right lane, respectively, are Hct116 (human colon cancer cells), Skov3 (ovarian carcinoma cells), and HTR-8/SVneo. Hct116 and Skov3 were used as control. GAPDH was used as protein control to ensure an equal amount of loaded protein. (c) Effects of IL-27 (50 ng/mL) on the mRNA expression of inflammatory mediators. Kinetic gene expressed of CXCL10 (d) and IL-6 (e) in HTR-8/SVneo cells under stimulated with IL-27. * P < 0.05, ** P < 0.01, and *** P < 0.001 when compared between groups denoted by horizontal lines ( n = 3). (f) CXCL10 protein levels after the stimulation of IL-27 (0–50 ng/mL) in cell supernatant of HTR-8/SVneo (0–30 h). (g) IL-6 protein expression after the stimulation of IL-27 (0–50 ng/mL) in cell supernatant of HTR-8/SVneo (0–48 h). * P < 0.05, ** P < 0.01, and *** P < 0.001 when comparing the level at the starting time point. All experiments were performed in three independent replicates.

Article Snippet: Sections were incubated with anti-IL-27 mAb (Abcam115671, HK) and anti-WSX-1 mAb (RD AF1479), diluted in 5% (wt/vol) nonfat milk for 16 h at 4°C.

Techniques: Expressing